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KMID : 0382619880080020745
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Hanyang Journal of Medicine
1988 Volume.8 No. 2 p.745 ~ p.755
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Acid and Neutral Ribonucleases in Serum of Patients. with Lymphocytic and Myelogenous Leukemia
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Abstract
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Activities and positive rates of acid and neutral ribonucleases (RNases) were deter-mined in serum of partients with acute lymphocytic and myelogenous leukemia (ALL and AML) to investigate whether these serum enzymes could be used as a biochemical marker for leukemia. Also studied were presence of proteins.and RNases unique to luekemia and common to both ALL and AML, fractionating leukemic serum by a DEAE-cellulose column chromatography and polyacrylamide gel electrophoresis (PAGE).
(1) Activities of serum acid.and neutral RNases were significantly increased in both ALL and AML,. the degree of .increment´ in the activity and positive rate being greater in AML than ALL. The resultss suggested that the measurement of serum RNase activity could be used more effectively as a diagnostic ,marker for AML rather than ALL.
(2) Both acid and neutral RNases in serum were fractionated into 4 peaks respec-tively in ALL and AML by a DEAE-cellulose column chromatography. Chromatographic patterns for acid (peak Ii and III) and neutral (III) RNases of ALL and for acid (peak I) and neutral (peak I) RNases of AML were observed to be quite different from those of control, suggesting the possible existance of RNase unique to leukemia.
(3) Native PAGE´ analyses of DEAE-cellulose peak proteins revealed that protein band 8 in peak I, bands 4 and 7 in peak III, and bands 5 and 7 in peak IV were found to be specific to leukemia and common to both ALL and AML. These results in-dicated that proteins unique to leukemia were present in multiple in both ALL and AML.
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KEYWORD
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